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normal human epidermal kcs collected from neonatal foreskin  (Kurabo industries)

 
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    Kurabo industries normal human epidermal kcs collected from neonatal foreskin
    Normal Human Epidermal Kcs Collected From Neonatal Foreskin, supplied by Kurabo industries, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+epidermal+kcs/normal+human+epidermal+kcs+collected+from+neonatal+foreskin/bio_rxiv__2024__11__12__623158-105-6-11
    Average 90 stars, based on 1 article reviews
    normal human epidermal kcs collected from neonatal foreskin - by Bioz Stars, 2026-09
    90/100 stars

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    Cell Culture:

    Article Title: E-cadherin interactions are required for Langerhans cell differentiation
    Article Snippet: Then LCs were selected from layered cells on a Ficoll gradient using CD1a-labeled immunomagnetic microbeads (Miltenyi Biotec, Aubum, CA, USA). .. Human epidermal KCs (Kurabo, Osaka, Japan) were maintained in KC culture medium (EpiLfe-KG2; Kurabo), and then cultured KCs were plated in 24-well culture plates and further cultured until confluence. .. PBMo-derived LC-like cells were then loaded onto KC culture plates at a concentration of 1 × 10 5 cell/mL in CCM supplemented with 100 ng/mL GM-CSF, with or without polycarbonate membrane inserts (Corning, NY, USA).



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    Epidermal thickness and the expression of GCSF, CCL20, and tyrosinase protein in UVB-treated mouse skin. Epidermal thickness ( A ), immunofluorescence of GCSF ( A ), CCL20 ( C ), tyrosinase ( A , C ), and pan-cytokeratin, a marker that identifies <t>keratinocytes</t> in all layers of the epidermis ( A , C ), were assessed 12 h following the final UVB exposure. Red arrows indicate the epidermal layer, while white arrows mark the dermal-epidermal junction, delineating the boundary between the epidermis and dermis. The summary graph shows the statistical analysis of epidermal thickness ( B ), and the relative protein levels of GCSF ( D ), and CCL20 ( E ), to pan-cytokeratin and tyrosinase ( F ). The data were quantified using ImageJ and GraphPad Prism software (v9) and are expressed as mean ± SD, with 1 N (dot) representing one area from four areas in a mouse. A mouse provides three N. The statistical significance of differences was evaluated by one-way ANOVA followed by Dunnett’s test. *** p < 0.001 versus non-irradiated group.
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    Image Search Results


    Epidermal thickness and the expression of GCSF, CCL20, and tyrosinase protein in UVB-treated mouse skin. Epidermal thickness ( A ), immunofluorescence of GCSF ( A ), CCL20 ( C ), tyrosinase ( A , C ), and pan-cytokeratin, a marker that identifies keratinocytes in all layers of the epidermis ( A , C ), were assessed 12 h following the final UVB exposure. Red arrows indicate the epidermal layer, while white arrows mark the dermal-epidermal junction, delineating the boundary between the epidermis and dermis. The summary graph shows the statistical analysis of epidermal thickness ( B ), and the relative protein levels of GCSF ( D ), and CCL20 ( E ), to pan-cytokeratin and tyrosinase ( F ). The data were quantified using ImageJ and GraphPad Prism software (v9) and are expressed as mean ± SD, with 1 N (dot) representing one area from four areas in a mouse. A mouse provides three N. The statistical significance of differences was evaluated by one-way ANOVA followed by Dunnett’s test. *** p < 0.001 versus non-irradiated group.

    Journal: Cells

    Article Title: Protective Effects of Keratinocyte-Derived GCSF and CCL20 on UVB-Induced Melanocyte Damage

    doi: 10.3390/cells13191661

    Figure Lengend Snippet: Epidermal thickness and the expression of GCSF, CCL20, and tyrosinase protein in UVB-treated mouse skin. Epidermal thickness ( A ), immunofluorescence of GCSF ( A ), CCL20 ( C ), tyrosinase ( A , C ), and pan-cytokeratin, a marker that identifies keratinocytes in all layers of the epidermis ( A , C ), were assessed 12 h following the final UVB exposure. Red arrows indicate the epidermal layer, while white arrows mark the dermal-epidermal junction, delineating the boundary between the epidermis and dermis. The summary graph shows the statistical analysis of epidermal thickness ( B ), and the relative protein levels of GCSF ( D ), and CCL20 ( E ), to pan-cytokeratin and tyrosinase ( F ). The data were quantified using ImageJ and GraphPad Prism software (v9) and are expressed as mean ± SD, with 1 N (dot) representing one area from four areas in a mouse. A mouse provides three N. The statistical significance of differences was evaluated by one-way ANOVA followed by Dunnett’s test. *** p < 0.001 versus non-irradiated group.

    Article Snippet: Primary human epidermal melanocytes (MC) and primary human epidermal keratinocytes (KC) were obtained from Invitrogen (Waltham, MA, USA).

    Techniques: Expressing, Immunofluorescence, Marker, Software, Irradiation